AP-4 complex subunit epsilon Rabbit Polyclonal Antibody
cat.: HA500446
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Arabidopsis thaliana |
| Applications: | IF-Tissue, IHC-P, ELISA |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 104 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within ARATH AP-4 complex subunit epsilon aa 880-938 / 938. |
| Positive control: | A. thaliana tissue. |
| Subcellular location: | Trans-Golgi network, coated pit. |
| Recommended Dilutions:
IF-Tissue IHC-P ELISA |
1:50-1:100 1:50-1:200 1:10,000 |
| Uniprot #: | SwissProt: Q8L7A9 ARATH |
| Alternative names: | AP-4 complex subunit epsilon AP-4 adaptor complex subunit epsilon Adaptor-related protein complex 4 subunit epsilon Epsilon subunit of AP-4 Epsilon-adaptin At1g31730 F27M3.7 |
Images
|
Fig1: Immunofluorescence staining of paraffin- embedded A. thaliana using anti-AP-4 complex subunit epsilon rabbit polyclonal antibody.The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with AP-4 complex subunit epsilon antibody at 1/50 dilution for 10 hours at 4℃ and detected using Alexa Fluor® 488 conjugate-Goat anti-Rabbit IgG (H+L) Secondary Antibody at a dilution of 1:500 for 1 hour at room temperature. |
|
Fig2: Immunohistochemical analysis of paraffin-embedded A. thaliana tissue using anti-AP-4 complex subunit epsilon antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500446, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.