AP-4 complex subunit mu Rabbit Polyclonal Antibody
cat.: HA500447
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Arabidopsis thaliana |
| Applications: | IF-Tissue, IHC-P, ELISA |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 51 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within A. thaliana AP-4 complex subunit mu aa 1-50 / 451. |
| Positive control: | A. thaliana tissue. |
| Subcellular location: | Trans-Golgi network, coated pit. |
| Recommended Dilutions:
IF-Tissue IHC-P ELISA |
1:50-1:100 1:50-1:200 1:10,000 |
| Uniprot #: | SwissProt: Q9SB50 ARATH |
| Alternative names: | AP-4 complex subunit mu Adaptor protein complex AP-4 subunit mu Adaptor protein-4 mu-adaptin Adaptor-related protein complex 4 subunit mu At-muC-Ad Mu4-adaptin AP4M CLH At4g24550 F22K18.250 |
Images
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Fig1: Immunofluorescence staining of paraffin- embedded A. thaliana using anti-AP-4 complex subunit mu rabbit polyclonal antibody.The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with AP-4 complex subunit mu antibody at 1/50 dilution for 10 hours at 4℃ and detected using Alexa Fluor® 488 conjugate-Goat anti-Rabbit IgG (H+L) Secondary Antibody at a dilution of 1:500 for 1 hour at room temperature. |
|
Fig2: Immunohistochemical analysis of paraffin-embedded A. thaliana tissue using anti-AP-4 complex subunit mu antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500447, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.