AP-4 complex subunit sigma Rabbit Polyclonal Antibody
cat.: HA500449
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Arabidopsis thaliana
Applications: IF-Tissue, IHC-P, ELISA
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: 17 kDa
Isotype: IgG
Immunogen: Synthetic peptide within A. thaliana AP-4 complex subunit sigma aa 1-50 / 143.
Positive control: A. thaliana tissue.
Subcellular location: Trans-Golgi network, coated pit.
Recommended Dilutions:
  IF-Tissue
  IHC-P
  ELISA

1:50-1:100
1:50-1:200
1:10,000
Uniprot #: SwissProt: O82201 ARATH
Alternative names: AP-4 complex subunit sigma AP-4 adaptor complex subunit sigma Adaptor-related protein complex 4 subunit sigma Sigma subunit of AP-4 Sigma4-adaptin At2g19790 F6F22.18
Images
HA500449_1.jpg Fig1: Immunofluorescence staining of paraffin- embedded A. thaliana using anti-AP-4 complex subunit sigma rabbit polyclonal antibody.The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with AP-4 complex subunit sigma antibody at 1/50 dilution for 10 hours at 4℃ and detected using Alexa Fluor® 488 conjugate-Goat anti-Rabbit IgG (H+L) Secondary Antibody at a dilution of 1:500 for 1 hour at room temperature.
HA500449_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded A. thaliana tissue using anti-AP-4 complex subunit sigma antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500449, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.