JAZ11 Rabbit Polyclonal Antibody
cat.: HA500450
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Rice |
| Applications: | ELISA, IF-Tissue, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 22 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within rice JAZ11 aa 160-209 / 209. |
| Positive control: | Rice tissue. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
ELISA IF-Tissue IHC-P |
1:10,000 1:50-1:100 1:50-1:200 |
| Uniprot #: | SwissProt: Q8GRS2 ORYSJ |
| Alternative names: | Protein TIFY 11c OsTIFY11c Jasmonate ZIM domain-containing protein 11 OsJAZ11 OsJAZ2 TIFY11C JAZ11 Os03g0180900 LOC_Os03g08320 OsJ_09657 OSJNBa0050H14.23 OSJNBb0076N15.9 |
Images
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Fig1: Immunofluorescence staining of paraffin- embedded rice tissue using anti-JAZ11 rabbit polyclonal antibody.The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with JAZ11 antibody at 1/50 dilution for 10 hours at 4℃ and detected using Alexa Fluor® 488 conjugate-Goat anti-Rabbit IgG (H+L) Secondary Antibody at a dilution of 1:500 for 1 hour at room temperature. |
|
Fig2: Immunohistochemical analysis of paraffin-embedded rice tissue using anti-JAZ11 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500450, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.