PSMA4 Rabbit Polyclonal Antibody
cat.: HA500452
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 29 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human PSMA4 aa 62-261. |
| Positive control: | Jurkat cell lysate, Hela cell lysate, K562 cell lysate, U937 cell lysate, rat testis tissue lysate, mouse cerebellum tissue lysate, human placenta tissue lysate, rat kidney tissue. |
| Subcellular location: | Cytoplasm. Nucleus. Cytoplasm, P-body. |
| Recommended Dilutions:
WB IHC-P |
1:1,000 1:800 |
| Uniprot #: | SwissProt: P25789 Human | Q9R1P0 Mouse | P21670 Rat |
| Alternative names: | HC9 HsT17706 Macropain subunit C9 MGC111191 MGC12467 MGC24813 Multicatalytic endopeptidase complex subunit C9 Proteasome (prosome macropain) subunit alpha type 4 Proteasome alpha 4 subunit Proteasome component C9 Proteasome subunit alpha type 4 Proteasome subunit alpha type-4 Proteasome subunit HC9 Proteasome subunit L PSA4_HUMAN PSC9 PSMA 4 psmA4 |
Images
|
Fig1:
Western blot analysis of PSMA4 on different lysates with Rabbit anti-PSMA4 antibody (HA500452) at 1/1,000 dilution. Lane 1: Jurkat cell lysate (10 µg/Lane) Lane 2: Hela cell lysate (10 µg/Lane) Lane 3: K562 cell lysate (10 µg/Lane) Lane 4: U937 cell lysate (10 µg/Lane) Lane 5: Rat testis tissue lysate (20 µg/Lane) Lane 6: Mouse cerebellum tissue lysate (20 µg/Lane) Lane 7: Human placenta tissue lysate (20 µg/Lane) Predicted band size: 29 kDa Observed band size: 29 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500452) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-PSMA4 antibody (HA500452) at 1/800 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500452) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.