IL-4R Rabbit Polyclonal Antibody
cat.: HA500453
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 90 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human IL-4R aa 651-700 / 825. |
| Positive control: | PC-3M cell lysates, PC-3M. |
| Subcellular location: | Cell membrane; Secreted. |
| Recommended Dilutions:
WB IF-Cell |
1:500 1:100 |
| Uniprot #: | SwissProt: P24394 Human |
| Alternative names: | CD124 IL 4R alpha IL-4 receptor subunit alpha IL-4-binding protein IL-4R subunit alpha IL-4R-alpha IL-4RA IL4-BP Il4r IL4R nirs variant 1 IL4RA IL4RA_HUMAN Interleukin 4 receptor alpha chain Interleukin 4 receptor sIL4Ralpha/prot Soluble IL-4 receptor subunit alpha Soluble IL-4R-alpha Soluble interleukin-4 receptor subunit alpha |
Images
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Fig1:
Western blot analysis of IL-4R on PC-3M cell lysates with Rabbit anti-IL-4R antibody (HA500453) at 1/500 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 90 kDa Observed band size: 140 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500453) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunocytochemistry analysis of PC-3M cells labeling IL-4R with Rabbit anti-IL-4R antibody (HA500453) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-IL-4R antibody (HA500453) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.