IL-4R Rabbit Polyclonal Antibody
cat.: HA500454
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2.6ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 90 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human IL-4R aa 101-150 / 825. |
| Positive control: | THP-1 cell lysate, 293T cell lysate, K562 cell lysate, Jurkat cell lysate, PC-12 cell lysate, MCF-7 cell lysate, human spleen tissue. |
| Subcellular location: | Cell membrane; Secreted. |
| Recommended Dilutions:
WB IHC-P |
1:500 1:600 |
| Uniprot #: | SwissProt: P24394 Human |
| Alternative names: | CD124 IL 4R alpha IL-4 receptor subunit alpha IL-4-binding protein IL-4R subunit alpha IL-4R-alpha IL-4RA IL4-BP Il4r IL4R nirs variant 1 IL4RA IL4RA_HUMAN Interleukin 4 receptor alpha chain Interleukin 4 receptor sIL4Ralpha/prot Soluble IL-4 receptor subunit alpha Soluble IL-4R-alpha Soluble interleukin-4 receptor subunit alpha |
Images
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Fig1:
Western blot analysis of IL-4R on different lysates with Rabbit anti-IL-4R antibody (HA500454) at 1/500 dilution. Lane 1: THP-1 cell lysate Lane 2: 293T cell lysate Lane 3: K562 cell lysate Lane 4: Jurkat cell lysate Lane 5: PC-12 cell lysate Lane 6: MCF-7 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 90 kDa Observed band size: 110/140kDa Exposure time: 2 minutes; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500454) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-IL-4R antibody (HA500454) at 1/600 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500454) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.