IL-4R Rabbit Polyclonal Antibody
cat.: HA500454
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IF-Tissue |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2.6ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 90 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human IL-4R aa 101-150 / 825. |
| Positive control: | THP-1 cell lysate, Daudi cell lysate, 293T cell lysate, K-562 cell lysate, Jurkat cell lysate, MCF7 cell lysate, Ramos cell lysate, PC-12 cell lysate, Mouse spleen tissue lysate, Mouse pancreas tissue lysate, Rat pancreas tissue lysate. |
| Subcellular location: | Cell membrane; Secreted. |
| Recommended Dilutions:
WB IHC-P IF-Tissue |
1:2,000 1:600 1:500 |
| Uniprot #: | SwissProt: P24394 Human | P16382 Mouse | Q63257 Rat |
| Alternative names: | CD124 IL 4R alpha IL-4 receptor subunit alpha IL-4-binding protein IL-4R subunit alpha IL-4R-alpha IL-4RA IL4-BP Il4r IL4R nirs variant 1 IL4RA IL4RA_HUMAN Interleukin 4 receptor alpha chain Interleukin 4 receptor sIL4Ralpha/prot Soluble IL-4 receptor subunit alpha Soluble IL-4R-alpha Soluble interleukin-4 receptor subunit alpha |
Images
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Fig1:
Western blot analysis of IL-4R on different lysates with Rabbit anti-IL-4R antibody (HA500454) at 1/2,000 dilution. Lane 1: THP-1 cell lysate (20 µg/Lane) Lane 2: Daudi cell lysate (20 µg/Lane) Lane 3: 293T cell lysate (20 µg/Lane) Lane 4: K-562 cell lysate (20 µg/Lane) Lane 5: Jurkat cell lysate (20 µg/Lane) Lane 6: MCF7 cell lysate (20 µg/Lane) Lane 7: Ramos cell lysate (20 µg/Lane) Lane 8: PC-12 cell lysate (20 µg/Lane) Lane 9: Mouse spleen tissue lysate (40 µg/Lane) Lane 10: Mouse pancreas tissue lysate (40 µg/Lane) Lane 11: Rat pancreas tissue lysate (40 µg/Lane) Predicted band size: 90 kDa Observed band size: 110 kDa Exposure time: 1 minute; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500454) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-IL-4R antibody (HA500454) at 1/600 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500454) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Application: IF-Tissue Species: Human Site: tonsil Sample: Paraffin-embedded section Antibody concentration: 1/500 |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.