WDR77 Rabbit Polyclonal Antibody
cat.: HA500456
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 37 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human WDR77 aa 1-342 / 342. |
| Positive control: | Hep G2 cell lysate, Daudi cell lysate, Hela cell lysate, HEK-293 cell lysate, K-562 cell lysate, A549 cell lysate, Hela, HepG2. |
| Subcellular location: | Nucleus, Cytoplasm. |
| Recommended Dilutions:
WB IF-Cell FC |
1:2,000-1:5,000 1:200 1:500-1:1,000 |
| Uniprot #: | SwissProt: Q9BQA1 Human |
| Alternative names: | 2610312E17Rik Androgen receptor cofactor p44 C79984 HKMT1069 MEP 50 MEP-50 MEP50 MEP50_HUMAN Methylosome protein 50 MGC2722 Nbla10071 p44 p44/Mep50 RGD1310479 RP11 552M11.3 WD repeat containing protein 77 WD repeat domain 77 WD repeat-containing protein 77 WDR77 |
Images
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Fig1:
Western blot analysis of WDR77 on different lysates with Rabbit anti-WDR77 antibody (HA500456) at 1/5,000 dilution. Lane 1: Hep G2 (Human hepatocellular carcinoma cell) cell lysate Lane 2: Daudi (Human Burkitt's lymphoma cell) cell lysate Lane 3: HeLa (Human cervical adenocarcinoma cell) cell lysate Lane 4: HEK-293 (Human embryonic kidney cell) cell lysate Lane 5: K-562 (Human chronic myelogenous leukemia cell) cell lysate Lane 6: A549 (Human lung adenocarcinoma cell) cell lysate Lysates/proteins at 15 µg/Lane. Exposure time: 6 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA500456, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 36.7 kDa Observed band size: 40 kDa |
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Fig2:
Immunocytochemistry analysis of Hela cells labeling WDR77 with Rabbit anti-WDR77 antibody (HA500456) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-WDR77 antibody (HA500456) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. |
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Fig3:
Flow cytometric analysis of HepG2 cells labeling WDR77. Cells were fixed and permeabilized. Then stained with the primary antibody (HA500456, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
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Fig4:
Flow cytometric analysis of Hela cells labeling WDR77. Cells were fixed and permeabilized. Then stained with the primary antibody (HA500456, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.