PPP2R5E Rabbit Polyclonal Antibody
cat.: HA500457
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IF-Cell, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1.74ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 55/46 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human PPP2R5E aa 1-200 / 467. |
| Positive control: | Human cervical carcinoma tissue, mouse stomach tissue, rat esophagus tissue, MCF-7, MCF7 cell lysate, NIH/3T3 cell lysate, C6 cell lysate. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB IHC-P IF-Cell FC |
1:1,000 1:400 1:200 1:500-1:1,000 |
| Uniprot #: | SwissProt: Q16537 Human | Q61151 Mouse Entrez Gene: 299147 Rat |
| Alternative names: | 2A5E_HUMAN Epsilon isoform of regulatory subunit B56 protein phosphatase 2A PP2A B subunit B' epsilon PP2A B subunit B' epsilon isoform PP2A B subunit B56 epsilon PP2A B subunit B56 epsilon isoform PP2A B subunit isoform B''-epsilon PP2A B subunit isoform B'-epsilon PP2A B subunit isoform B56-epsilon PP2A B subunit isoform PR61-epsilon PP2A B subunit isoform R5-epsilon PP2A B subunit PR61 epsilon PP2A B subunit PR61 epsilon isoform PP2A B subunit R5 epsilon PP2A B subunit R5 epsilon isoform PPP2R5E Protein phosphatase 2 regulatory subunit B (B56) epsilon isoform Protein phosphatase 2 regulatory subunit B' epsilon Protein phosphatase 2 regulatory subunit B' epsilon isoform Regulatory subunit B of protein phosphatase 2 epsilon isoform Serine/threonine protein phosphatase 2A 56 kDa regulatory subunit epsilon Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit epsilon isoform |
Images
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Fig1:
Western blot analysis of PPP2R5E on different lysates with Rabbit anti-PPP2R5E antibody (HA500457) at 1/1,000 dilution. Lane 1: MCF7 cell lysate Lane 2: NIH/3T3 cell lysate Lane 3: C6 cell lysate Lysates/proteins at 20 µg/Lane. Exposure time: 10 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA500457, 1/1,000 in 5% NFDM/TBST, overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1006), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 55/46 kDa Observed band size: 55/46 kDa |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human cervical carcinoma tissue with Rabbit anti-PPP2R5E antibody (HA500457) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500457) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded mouse stomach tissue with Rabbit anti-PPP2R5E antibody (HA500457) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500457) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded rat esophagus tissue with Rabbit anti-PPP2R5E antibody (HA500457) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500457) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunocytochemistry analysis of MCF-7 cells labeling PPP2R5E with Rabbit anti-PPP2R5E antibody (HA500457) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-PPP2R5E antibody (HA500457) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. |
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Fig6:
Flow cytometric analysis of MCF-7 cells labeling PPP2R5E. Cells were fixed and permeabilized. Then stained with the primary antibody (HA500457, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
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