PPP2R5E Rabbit Polyclonal Antibody
cat.: HA500457
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P, IF-Cell, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1.74ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 55 kDa
Isotype: IgG
Immunogen: Recombinant protein within Human PPP2R5E aa 1-200 / 467.
Positive control: Hela cell lysate, Daudi cell lysate, MCF-7 cell lysate, NIH/3T3 cell lysate, human skeletal muscle tissue lysate, mouse thymus tissue lysate, rat esophagus tissue, mouse stomach tissue, human cervical carcinoma tissue, MCF-7.
Subcellular location: Cytoplasm.
Recommended Dilutions:
  WB
  IHC-P
  IF-Cell
  FC
1:1,000
1:400
1:200
1:500-1:1,000
Uniprot #: SwissProt: Q16537 Human | Q61151 Mouse
Entrez Gene: 299147 Rat
Alternative names: 2A5E_HUMAN Epsilon isoform of regulatory subunit B56 protein phosphatase 2A PP2A B subunit B' epsilon PP2A B subunit B' epsilon isoform PP2A B subunit B56 epsilon PP2A B subunit B56 epsilon isoform PP2A B subunit isoform B''-epsilon PP2A B subunit isoform B'-epsilon PP2A B subunit isoform B56-epsilon PP2A B subunit isoform PR61-epsilon PP2A B subunit isoform R5-epsilon PP2A B subunit PR61 epsilon PP2A B subunit PR61 epsilon isoform PP2A B subunit R5 epsilon PP2A B subunit R5 epsilon isoform PPP2R5E Protein phosphatase 2 regulatory subunit B (B56) epsilon isoform Protein phosphatase 2 regulatory subunit B' epsilon Protein phosphatase 2 regulatory subunit B' epsilon isoform Regulatory subunit B of protein phosphatase 2 epsilon isoform Serine/threonine protein phosphatase 2A 56 kDa regulatory subunit epsilon Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit epsilon isoform
Images
HA500457_1.jpg Fig1: Western blot analysis of PPP2R5E on different lysates with Rabbit anti-PPP2R5E antibody (HA500457) at 1/1,000 dilution.

Lane 1: Hela cell lysate (10 µg/Lane)
Lane 2: Daudi cell lysate (10 µg/Lane)
Lane 3: MCF-7 cell lysate (10 µg/Lane)
Lane 4: NIH/3T3 cell lysate (10 µg/Lane)
Lane 5: Human skeletal muscle tissue lysate (20 µg/Lane)
Lane 6: Mouse thymus tissue lysate (20 µg/Lane)

Lysates/proteins at 10 µg/Lane.

Predicted band size: 55 kDa
Observed band size: 55/48 kDa

Exposure time: 2 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500457) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
HA500457_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded rat esophagus tissue with Rabbit anti-PPP2R5E antibody (HA500457) at 1/400 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500457) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA500457_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded mouse stomach tissue with Rabbit anti-PPP2R5E antibody (HA500457) at 1/400 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500457) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA500457_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human cervical carcinoma tissue with Rabbit anti-PPP2R5E antibody (HA500457) at 1/400 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500457) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA500457_5.jpg Fig5: Immunocytochemistry analysis of MCF-7 cells labeling PPP2R5E with Rabbit anti-PPP2R5E antibody (HA500457) at 1/200 dilution.

Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-PPP2R5E antibody (HA500457) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
HA500457_6.jpg Fig6: Flow cytometric analysis of MCF-7 cells labeling PPP2R5E.

Cells were fixed and permeabilized. Then stained with the primary antibody (HA500457, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.