PSMB3 Rabbit Polyclonal Antibody
cat.: HA500458
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 23 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant full length protein within human PSMB3. |
| Positive control: | Hela cell lysate, K562 cell lysate, 293T cell lysate, Jurkat cell lysate, Hela, Human skin tissue, Human breast tissue. |
| Subcellular location: | Cytoplasm, Nucleus, Proteasome |
| Recommended Dilutions:
WB IF-Cell IHC-P |
1:1,000 1:200 1:600 |
| Uniprot #: | SwissProt: P49720 Human |
| Alternative names: | C10 II HC10 II proteasome (prosome, macropain) subunit, beta type 3 Proteasome beta 3 subunit Proteasome chain 13 proteasome component C10 II Proteasome component C10-II Proteasome subunit beta type-3 Proteasome theta chain PSB3_HUMAN PSMB3 |
Images
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Fig1:
Western blot analysis of PSMB3 on different lysates with Rabbit anti-PSMB3 antibody (HA500458) at 1/1,000 dilution. Lane 1: Hela cell lysate, 10 µg/Lane Lane 2: K562 cell lysate, 10 µg/Lane Lane 3: 293T cell lysate, 10 µg/Lane Lane 4: Jurkat cell lysate, 10 µg/Lane Predicted band size: 23 kDa Observed band size: 23 kDa Exposure time: 2 minutes; Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500458) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. Rabbit anti-GAPDH antibody (ET1601-4) was used as loading control antibody at 1:10,000 dilution. |
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Fig2:
Immunocytochemistry analysis of Hela cells labeling PSMB3 with Rabbit anti-PSMB3 antibody (HA500458) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.05% Triton X-100 in PBS for 15 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-PSMB3 antibody (HA500458) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Mouse anti-Beta tubulin Antibody (M1305-2) was used as Microtubule Marker at 1/200 dilution and Goat Anti-Mouse IgG H&L (iFluor™ 647, HA1127) was used as the secondary antibody at 1/1,000 dilution. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded Human skin tissue with Rabbit anti-PSMB3 antibody (HA500458) at 1/600 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500458) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded Human breast tissue with Rabbit anti-PSMB3 antibody (HA500458) at 1/600 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500458) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.