ASC1 Rabbit Polyclonal Antibody
cat.: HA500460
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Rat |
| Applications: | WB |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 66 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human ASC1 aa 1-581 / 581. |
| Positive control: | A549 cell lysate, Hela cell lysate, U87MG cell lysate, 293T cell lysate, HL-60 cell lysate, HepG2 cell lysate, rat liver tissue lysates. |
| Subcellular location: | Nucleus, Cytoplasm, cytosol, Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: Q15650 Human Entrez Gene: 315769 Rat |
| Alternative names: | Activating signal cointegrator 1 ASC 1 ASC-1 HsT17391 Thyroid hormone receptor interactor 4 Thyroid receptor interacting protein 4 Thyroid receptor-interacting protein 4 TR-interacting protein 4 TRIP 4 TRIP-4 Trip4 TRIP4_HUMAN ZC2HC5 Zinc finger C2HC5 type |
Images
|
Fig1:
Western blot analysis of ASC1 on different lysates with Rabbit anti-ASC1 antibody (HA500460) at 1/1,000 dilution. Lane 1: A549 cell lysate Lane 2: Hela cell lysate Lane 3: U87MG cell lysate Lane 4: 293T cell lysate Lane 5: HL-60 cell lysate Lane 6: HepG2 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 66 kDa Observed band size: 66 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500460) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of ASC1 on rat liver tissue lysates with Rabbit anti-ASC1 antibody (HA500460) at 1/1,000 dilution. Lysates/proteins at 20 µg/Lane. Predicted band size: 66 kDa Observed band size: 66 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500460) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.