AIMP2 Rabbit Polyclonal Antibody
cat.: HA500461
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IF-Cell |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 35 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human AIMP2 aa 71-320 / 320. |
| Positive control: | Hela cell lysate, Jurkat cell lysate, A549 cell lysate, SH-SY5Y cell lysate, human kidney tissue lysate, rat kidney tissue lysate, mouse kidney tissue lysate, human testis tissue, mouse kidney tissue, SH-SY5Y. |
| Subcellular location: | Cytoplasm, cytosol, Nucleus. |
| Recommended Dilutions:
WB IHC-P IF-Cell |
1:1,000 1:4,000 1:200 |
| Uniprot #: | SwissProt: Q13155 Human | Q8R010 Mouse | Q32PX2 Rat |
| Alternative names: | Aimp2 AIMP2_HUMAN Aminoacyl tRNA synthase complex-interacting multifunctional protein 2 Aminoacyl tRNA synthetase complex interacting multifunctional protein 2 ARS interacting multi functional protein 2 JTV 1 JTV 1 protein JTV1 JTV1 gene Multisynthase complex auxiliary component p38 Multisynthetase complex auxiliary component p38 P38 PRO0992 Protein JTV 1 Protein JTV-1 tRNA SYNTHETASE COFACTOR p38 |
Images
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Fig1:
Western blot analysis of AIMP2 on different lysates with Rabbit anti-AIMP2 antibody (HA500461) at 1/1,000 dilution. Lane 1: Hela cell lysate (10 µg/Lane) Lane 2: Jurkat cell lysate (10 µg/Lane) Lane 3: A549 cell lysate (10 µg/Lane) Lane 4: SH-SY5Y cell lysate (10 µg/Lane) Lane 5: Human kidney tissue lysate (20 µg/Lane) Lane 6: Rat kidney tissue lysate (20 µg/Lane) Lane 7: Mouse kidney tissue lysate (20 µg/Lane) Predicted band size: 35 kDa Observed band size: 40 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500461) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-AIMP2 antibody (HA500461) at 1/4,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500461) at 1/4,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-AIMP2 antibody (HA500461) at 1/4,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500461) at 1/4,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunocytochemistry analysis of SH-SY5Y cells labeling AIMP2 with Rabbit anti-AIMP2 antibody (HA500461) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-AIMP2 antibody (HA500461) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.