CPN1 Rabbit Polyclonal Antibody
cat.: HA500462
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IF-Cell |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 52 kDa (Predicted band size) |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human CPN1 aa 1-200 / 458. |
| Positive control: | Hela cell lysate, Raji cell lysate, mouse pancreas tissue lysate, LOVO. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
WB IF-Cell |
1:500 1:50-1:200 |
| Uniprot #: | SwissProt: P15169 Human | Q9JJN5 Mouse |
| Alternative names: | ACBP Anaphylatoxin inactivator Arginine carboxypeptidase Carboxypeptidase N catalytic chain Carboxypeptidase N polypeptide 1 50 kD Carboxypeptidase N polypeptide 1 Carboxypeptidase N small subunit Carboxypeptidase N subunit 1 CBPN_HUMAN CPN CPN1 Kininase 1 Kininase-1 Kininase1 Lysine carboxypeptidase Plasma carboxypeptidase B SCPN Serum carboxypeptidase N |
Images
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Fig1: ICC staining of CPN1 in LOVO cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (HA500462, 1/100) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
|
Fig2:
Western blot analysis of CPN1 on MCF7 cell lysate with Rabbit anti-CPN1 antibody (HA500462) at 1/1,000 dilution. Lysates/proteins at 20 µg/Lane. Exposure time: 120 seconds; ECL: K1802 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA500462, 1/1,000 in 5% NFDM/TBST, overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 52 kDa Observed band size: 60 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.