Osteopontin Rabbit Polyclonal Antibody
cat.: HA500464
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 32 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within mouse steopontin aa 1-50 / 314. |
| Positive control: | Mouse liver tissue lysate, HepG2 cell lysate, 213. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
WB FC |
1:500 1:50-1:100 |
| Uniprot #: | SwissProt: P10451 Human | P10923 Mouse |
| Alternative names: | BNSP Bone sialoprotein 1 BSP I BSPI Early T lymphocyte activation 1 ETA 1 ETA1 MGC110940 Nephropontin OPN Osteopontin osteopontin/immunoglobulin alpha 1 heavy chain constant region fusion protein OSTP_HUMAN PSEC0156 secreted phosphoprotein 1 (osteopontin, bone sialoprotein I, early T-lymphocyte activation 1) Secreted phosphoprotein 1 SPP 1 SPP-1 SPP1 SPP1/CALPHA1 fusion Urinary stone protein Uropontin |
Images
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Fig1:
Western blot analysis of Osteopontin on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500464, 1/500) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Mouse liver tissue lysate Lane 2: HepG2 cell lysate |
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Fig2: Flow cytometric analysis of Osteopontin was done on 213 cells. The cells were fixed, permeabilized and stained with the primary antibody (HA500464, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.