CNOT1 Rabbit Polyclonal Antibody
cat.: HA500466
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Rat, Mouse |
| Applications: | WB, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 267/241/174 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human CNOT1 aa 1-200 / 2376. |
| Positive control: | Rat brain tissue lysates, SiHa. |
| Subcellular location: | Cytoplasm, Nucleus. |
| Recommended Dilutions:
WB FC |
1:500-1:1,1000 1:100-1:500 |
| Uniprot #: | SwissProt: A5YKK6 Human | G3V7M0 Rat |
| Alternative names: | adrenal gland protein AD-005 CCR4 NOT transcription complex subunit 1 isoform b CCR4-associated factor 1 hNOT1 Negative regulator of transcription subunit 1 homolog NOT1 (negative regulator of transcription 1 yeast) homolog NOT1H |
Images
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Fig1: Western blot analysis of CNOT1 on rat brain tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500466, 1/500) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. |
|
Fig2: Flow cytometric analysis of CNOT1 was done on SiHa cells. The cells were fixed, permeabilized and stained with the primary antibody (HA500466, 1ug/ml) (red) compared with Rabbit IgG, monoclonal - Isotype Control (green). After incubation of the primary antibody at +4℃ for 1 hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃ (dark incubation).Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.