ADX Rabbit Polyclonal Antibody
cat.: HA500468
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1.5ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 19 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human ADX aa 50-184. |
| Positive control: | SiHa cell lysate, SK-Br-3 cell lysate, K562 cell lysate, HepG2 cell lysate, 293T cell lysate, mouse liver tissue lysate, rat liver tissue lysate, rat testis tissue lysate. |
| Subcellular location: | Mitochondrion matrix. |
| Recommended Dilutions:
WB |
1:500 |
| Uniprot #: | SwissProt: P10109 Human | P46656 Mouse | P24483 Rat |
| Alternative names: | Adrenal ferredoxin Adrenodoxin Adrenodoxin, mitochondrial ADX ADX_HUMAN FDX fdx1 Ferredoxin 1 Ferredoxin-1 Hepatoredoxin LOH11CR1D Mitochondrial adrenodoxin mitochondrial |
Images
|
Fig1:
Western blot analysis of ADX on different lysates with Rabbit anti-ADX antibody (HA500468) at 1/500 dilution. Lane 1: SiHa cell lysate (15 µg/Lane) Lane 2: SK-Br-3 cell lysate (15 µg/Lane) Lane 3: K562 cell lysate (15 µg/Lane) Lane 4: HepG2 cell lysate (15 µg/Lane) Lane 5: 293T cell lysate (15 µg/Lane) Lane 6: Mouse liver tissue lysate (30 µg/Lane) Lane 7: Rat liver tissue lysate (30 µg/Lane) Lane 8: Rat testis tissue lysate (30 µg/Lane) Predicted band size: 19 kDa Observed band size: 13 kDa Exposure time: 2 minutes; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500468) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.