Cathepsin K Rabbit Polyclonal Antibody
cat.: HA500469
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 37 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Cathepsin K aa 101-250 / 329. |
| Positive control: | Jurkat cell lysate, K562 cell lysate, A549 cell lysate, U87MG cell lysate, A431 cell lysate, Jurkat. |
| Subcellular location: | Lysosome, Secreted, Apical cell membrane. |
| Recommended Dilutions:
WB IF-Cell |
1:500 1:100 |
| Uniprot #: | SwissProt: P43235 Human |
| Alternative names: | Cathepsin K Cathepsin O Cathepsin O1 Cathepsin O2 Cathepsin X CATK_HUMAN CTS02 Ctsk CTSO CTSO1 CTSO2 MGC23107 PKND PYCD |
Images
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Fig1:
Western blot analysis of Cathepsin K on different lysates with Rabbit anti-Cathepsin K antibody (HA500469) at 1/500 dilution. Lane 1: Jurkat cell lysate Lane 2: K562 cell lysate Lane 3: A549 cell lysate Lane 4: U87MG cell lysate Lane 5: A431 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 37 kDa Observed band size: 27 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500469) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunocytochemistry analysis of Jurkat cells labeling Cathepsin K with Rabbit anti-Cathepsin K antibody (HA500469) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Cathepsin K antibody (HA500469) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.