Cathepsin K Rabbit Polyclonal Antibody
cat.: HA500469
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 37 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Cathepsin K aa 101-250 / 329. |
| Positive control: | Jurkat cell lysate, K562 cell lysate, A549 cell lysate, U87MG cell lysate, A431 cell lysate. |
| Subcellular location: | Lysosome, Secreted, Apical cell membrane. |
| Recommended Dilutions:
WB |
1:500 |
| Uniprot #: | SwissProt: P43235 Human |
| Alternative names: | Cathepsin K Cathepsin O Cathepsin O1 Cathepsin O2 Cathepsin X CATK_HUMAN CTS02 Ctsk CTSO CTSO1 CTSO2 MGC23107 PKND PYCD |
Images
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Fig1:
Western blot analysis of Cathepsin K on different lysates with Rabbit anti-Cathepsin K antibody (HA500469) at 1/500 dilution. Lane 1: Jurkat cell lysate Lane 2: K562 cell lysate Lane 3: A549 cell lysate Lane 4: U87MG cell lysate Lane 5: A431 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 37 kDa Observed band size: 27 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500469) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.