| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 22 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human MYL4 aa 10-197 / 197. |
| Positive control: | Mouse lung tissue lysate, Rat lung tissue lysate. |
| Subcellular location: | Myosin complex, cytosol, A band. |
| Recommended Dilutions:
WB |
1:500-1:2,000 |
| Uniprot #: | SwissProt: P12829 Human | P09541 Mouse | P17209 Rat |
| Alternative names: | ALC 1 ALC1 AMLC Atrial/embryonic alkali myosin light chain embryonic muscle/atrial isoform GT 1 GT1 MLC 1 MLC1 MYL 4 Myl4 MYL4_HUMAN Myosin atrial/fetal muscle light chain Myosin light chain 1 Myosin light chain 1 embryonic muscle/atrial isoform Myosin light chain 4 alkali atrial embryonic Myosin light chain 4 Myosin light chain alkali GT 1 isoform Myosin light chain alkali GT-1 isoform Myosin light polypeptide 4 alkali atrial embryonic Myosin light polypeptide 4 PRO1957 |
|
Fig1:
Western blot analysis of MYL4 on different lysates with Rabbit anti-MYL4 antibody (HA500471) at 1/5,000 dilution. Lane 1: Mouse lung tissue lysate Lane 2: Rat lung tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 22 kDa Observed band size: 22 kDa Exposure time: 2 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500471) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |