MYL4 Rabbit Polyclonal Antibody
cat.: HA500471
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB
Clonality: Polyclonal
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage buffer: 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 22 kDa
Isotype: IgG
Immunogen: Recombinant protein within human MYL4 aa 10-197 / 197.
Positive control: Mouse lung tissue lysate, Rat lung tissue lysate.
Subcellular location: Myosin complex, cytosol, A band.
Recommended Dilutions:
  WB

1:500-1:2,000
Uniprot #: SwissProt: P12829 Human | P09541 Mouse | P17209 Rat
Alternative names: ALC 1 ALC1 AMLC Atrial/embryonic alkali myosin light chain embryonic muscle/atrial isoform GT 1 GT1 MLC 1 MLC1 MYL 4 Myl4 MYL4_HUMAN Myosin atrial/fetal muscle light chain Myosin light chain 1 Myosin light chain 1 embryonic muscle/atrial isoform Myosin light chain 4 alkali atrial embryonic Myosin light chain 4 Myosin light chain alkali GT 1 isoform Myosin light chain alkali GT-1 isoform Myosin light polypeptide 4 alkali atrial embryonic Myosin light polypeptide 4 PRO1957
Images
HA500471_1.jpg Fig1: Western blot analysis of MYL4 on different lysates with Rabbit anti-MYL4 antibody (HA500471) at 1/5,000 dilution.

Lane 1: Mouse lung tissue lysate
Lane 2: Rat lung tissue lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 22 kDa
Observed band size: 22 kDa

Exposure time: 2 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500471) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.