MYL4 Rabbit Polyclonal Antibody
cat.: HA500471
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 22 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human MYL4 aa 10-197 / 197. |
| Positive control: | Mouse lung tissue lysate, human heart tissue lysate, rat lung tissue, rat heart tissue. |
| Subcellular location: | Myosin complex, cytosol, A band. |
| Recommended Dilutions:
WB IHC-P |
1:500-1:2,000 1:200-1:1,000 |
| Uniprot #: | SwissProt: P12829 Human | P09541 Mouse | P17209 Rat |
| Alternative names: | ALC 1 ALC1 AMLC Atrial/embryonic alkali myosin light chain embryonic muscle/atrial isoform GT 1 GT1 MLC 1 MLC1 MYL 4 Myl4 MYL4_HUMAN Myosin atrial/fetal muscle light chain Myosin light chain 1 Myosin light chain 1 embryonic muscle/atrial isoform Myosin light chain 4 alkali atrial embryonic Myosin light chain 4 Myosin light chain alkali GT 1 isoform Myosin light chain alkali GT-1 isoform Myosin light polypeptide 4 alkali atrial embryonic Myosin light polypeptide 4 PRO1957 |
Images
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Fig1:
Western blot analysis of MYL4 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500471, 1/500) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Mouse lung tissue lysate Lane 2: Human heart tissue lysate |
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Fig2: Immunohistochemical analysis of paraffin-embedded rat lung tissue using anti-MYL4 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500471, 1/800) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3: Immunohistochemical analysis of paraffin-embedded rat heart tissue using anti-MYL4 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500471, 1/800) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.