PFKP Rabbit Polyclonal Antibody
cat.: HA500472
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 86 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human PFKP aa 601-784 / 784. |
| Positive control: | A549 cell lysate, mouse lung tissue lysate, rat lung tissue lysate, human prostate carcinoma tissue, mouse brain tissue. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB IHC-P |
1:1,000-1:5,000 1:50-1:200 |
| Uniprot #: | SwissProt: Q01813 Human | Q9WUA3 Mouse | P47860 Rat |
| Alternative names: | 1200015H23Rik 6 phosphofructokinase type C 6 phosphofructokinase, platelet type 6-phosphofructokinase 6-phosphofructokinase type C 9330125N24Rik FLJ40226 K6PP K6PP_HUMAN MGC105718 PFK C PFK, fibroblast type PFK-C PFKF PFKP Phosphofructo 1 kinase isozyme C Phosphofructo-1-kinase isozyme C Phosphofructokinase 1 Phosphofructokinase platelet Phosphohexokinase platelet type |
Images
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Fig1:
Western blot analysis of PFKP on different lysates with Rabbit anti-PFKP antibody (HA500472) at 1/5,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-PFKP KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 86 kDa Observed band size: 86 kDa Exposure time: 12 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500472) at 1/5,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of PFKP on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500472, 1/5,000) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: A549 cell lysate Lane 2: Mouse lung tissue lysate Lane 3: Rat lung tissue lysate |
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Fig3: Immunohistochemical analysis of paraffin-embedded human prostate carcinoma tissue using anti-PFKP antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500472, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-PFKP antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500472, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.