GABRA6 Rabbit Polyclonal Antibody
cat.: HA500473
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 51 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human GABRA6 aa 43-242 / 453. |
| Positive control: | PC-3 cell lysate, rat brain tissue, mouse brain tissue. |
| Subcellular location: | Postsynaptic cell membrane, Cell membrane. |
| Recommended Dilutions:
WB IHC-P |
1:500-1:2,000 1:100-1:500 |
| Uniprot #: | SwissProt: Q16445 Human | P16305 Mouse | P30191 Rat |
| Alternative names: | GABA A GABA A Receptor alpha 6 polypeptide GABA A receptor alpha 6 GABA A receptor subunit alpha 6 GABA subunit A receptor alpha 6 GABA(A) receptor subunit alpha-6 GABRA 6 GABRA6 Gamma aminobutyric acid A receptor alpha 6 Gamma aminobutyric acid GABA A receptor alpha 6 Gamma aminobutyric acid receptor subunit alpha 6 Gamma-aminobutyric acid receptor subunit alpha-6 GBRA6_HUMAN MGC116903 MGC116904 |
Images
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Fig1:
Western blot analysis of GABRA6 on PC-3 (Human prostate cancer cell) cell lysate with Rabbit anti-GABRA6 antibody (HA500473) at 1/1,000 dilution. Exposure time: 180 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA500473, 1/1,000 in 5% NFDM/TBST, overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 51 kDa Observed band size: 51 kDa |
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Fig2: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-GABA A Receptor alpha 6 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500473, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-GABRA6 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500473, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.