TRIM33 Rabbit Polyclonal Antibody
cat.: HA500475
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 123 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human TRIM33 aa 900-1127 / 1127. |
| Positive control: | K562 cell lysates, mouse uterus tissue lysates, human placenta tissue, human thyroid tissue. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB IHC-P |
1:500-1:2,000 1:50-1:200 |
| Uniprot #: | SwissProt: Q9UPN9 Human | Q99PP7 Mouse |
| Alternative names: | 8030451N04Rik AI413936 cb1085 DKFZp586K1123 E3 ubiquitin-protein ligase TRIM33 EC 6.3.2.- Ectodermin Ectodermin homolog FLJ11429 FLJ32925 id:ibd2175 MGC136680 mKIAA1113 OTTHUMP00000013662 OTTHUMP00000013663 Protein Rfg7 PTC7 Ret fused gene 7 RET-fused gene 7 protein RFG7 Rfg7 protein TF1G TIF1 gamma TIF1-gamma TIF1G TIF1GAMMA TIFGAMMA Transcription intermediary factor 1-gamma Transcriptional intermediary factor 1 gamma TRI33_HUMAN trim33 Tripartite motif containing 33 Tripartite motif containing 33 protein tripartite motif-containing 33 Tripartite motif-containing protein 33 wu:fc17f10 zgc:136680 |
Images
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Fig1: Western blot analysis of TRIM33 on K562 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500475, 1/500) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. |
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Fig2: Western blot analysis of TRIM33 on mouse uterus tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500475, 1/2,000) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. |
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Fig3: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-TRIM33 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500475, 1/600) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4: Immunohistochemical analysis of paraffin-embedded human thyroid tissue using anti-TRIM33 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500475, 1/600) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.