SOX17 Rabbit Polyclonal Antibody
cat.: HA500476
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 44 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human SOX17 aa 1-50 / 414. |
| Positive control: | SKOV-3 cell lysate, HepG2 cell lysate, Hela cell lysate, mouse testis tissue lysate, rat testis tissue lysate, rat brain tissue lysate, Hela. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB FC |
1:500-1:2,000 1:500-1:1,000 |
| Uniprot #: | SwissProt: Q9H6I2 Human | Q61473 Mouse Entrez Gene: 312936 Rat |
| Alternative names: | FLJ22252 SOX17 SOX17_HUMAN SRY (sex determining region Y) box 17 SRY box 17 SRY related HMG box transcription factor SOX17 Transcription factor SOX-17 Transcription factor SOX17 VUR3 |
Images
|
Fig1:
Western blot analysis of SOX17 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500476, 1/1,000) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: SKOV-3 cell lysate Lane 2: HepG2 cell lysate Lane 3: Hela cell lysate Lane 4: Mouse testis tissue lysate Predicted band size: 44 kDa Observed band size: 55 kDa |
|
Fig2:
Western blot analysis of SOX17 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500476, 1/1,000) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Rat testis tissue lysate Lane 2: Rat brain tissue lysate Predicted band size: 44 kDa Observed band size: 55 kDa |
|
Fig3:
Flow cytometric analysis of Hela cells labeling SOX17. Cells were fixed and permeabilized. Then stained with the primary antibody (HA500476, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.