AP2A2 Rabbit Polyclonal Antibody
cat.: HA500477
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 104 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human AP2A2 aa 740-939 / 939. |
| Positive control: | Hela cell lysate, mouse cerebellum tissue lysate, rat brain tissue lysate, human kidney tissue, mouse cerebellum tissue. |
| Subcellular location: | Cell membrane, coated pit. |
| Recommended Dilutions:
WB IHC-P |
1:500-1:5,000 1:200-1:1,000 |
| Uniprot #: | SwissProt: O94973 Human | P17427 Mouse | P18484 Rat |
| Alternative names: | 100 kDa coated vesicle protein C Adaptor protein complex AP-2 subunit alpha-2 Adaptor-related protein complex 2 subunit alpha-2 Alpha-adaptin C Alpha2-adaptin AP-2 complex subunit alpha-2 Ap2a2 AP2A2_HUMAN Clathrin assembly protein complex 2 alpha-C large chain HIP-9 Huntingtin yeast partner J Huntingtin-interacting protein 9 Huntingtin-interacting protein J Plasma membrane adaptor HA2/AP2 adaptin alpha C subunit |
Images
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Fig1:
Western blot analysis of AP2A2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500477, 1/2,000) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 2: Hela cell lysate Lane 1: Mouse cerebellum tissue lysate Lane 1: Rat brain tissue lysate |
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Fig2: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-AP2A2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500477, 1/600) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3: Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue using anti-AP2A2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500477, 1/600) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.