NARS Rabbit Polyclonal Antibody
cat.: HA500479
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: 63 kDa
Isotype: IgG
Immunogen: Recombinant protein within human NARS aa 281-480 / 548.
Positive control: HepG2 cell lysate, K562 cell lysate, 293 cell lysate, NIH/3T3 cell lysate, mouse liver tissue lysate, mouse brain tissue, mouse cerebellum tissue, rat cerebellum tissue.
Subcellular location: Cytoplasm.
Recommended Dilutions:
  WB
  IHC-P
1:500-1:2,000
1:100-1:500
Uniprot #: SwissProt: O43776 Human | Q8BP47 Mouse
Entrez Gene: 291556 Rat
Alternative names: 3010001M15Rik AA960128 AsnRS ASNS Asparagine tRNA ligase 1, cytoplasmic Asparagine tRNA ligase Asparagine--tRNA ligase Asparaginyl tRNA synthetase Asparaginyl-tRNA synthetase C78150 cytoplasmic EC 6.1.1.22 LRRGT00113 MGC116236 Nars NARS1 NRS SYNC_HUMAN
Images
HA500479_1.jpg Fig1: Western blot analysis of NARS on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500479, 1/2,000) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: HepG2 cell lysate
Lane 2: K562 cell lysate
Lane 3: 293 cell lysate
HA500479_2.jpg Fig2: Western blot analysis of NARS on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500479, 1/1,000) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: NIH/3T3 cell lysate
Lane 2: Mouse liver tissue lysate
HA500479_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-NARS antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500479, 1/600) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA500479_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue using anti-NARS antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500479, 1/600) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA500479_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue using anti-NARS antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500479, 1/600) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.