NARS Rabbit Polyclonal Antibody
cat.: HA500479
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 63 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human NARS aa 281-480 / 548. |
| Positive control: | HepG2 cell lysate, K562 cell lysate, 293 cell lysate, NIH/3T3 cell lysate, Mouse liver tissue lysate. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB |
1:1,000-1:5,000 |
| Uniprot #: | SwissProt: O43776 Human | Q8BP47 Mouse Entrez Gene: 291556 Rat |
| Alternative names: | 3010001M15Rik AA960128 AsnRS ASNS Asparagine tRNA ligase 1, cytoplasmic Asparagine tRNA ligase Asparagine--tRNA ligase Asparaginyl tRNA synthetase Asparaginyl-tRNA synthetase C78150 cytoplasmic EC 6.1.1.22 LRRGT00113 MGC116236 Nars NARS1 NRS SYNC_HUMAN |
Images
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Fig1:
Western blot analysis of NARS on different lysates with Rabbit anti-NARS antibody (HA500479) at 1/5,000 dilution. Lane 1: K-562 (Human chronic myelogenous leukemia cell) cell lysate（20μg /Lane） Lane 2: HepG2 (Human hepatocellular carcinoma cell) cell lysate（20μg /Lane） Lane 3: Mouse liver tissue lysate（40μg /Lane） Lane 4: Rat brain tissue lysate（40μg /Lane） Lane 5: Rat liver tissue lysate（40μg /Lane） Exposure time: 46 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA500479, 1/5,000 in 5% NFDM/TBST, overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 63 kDa Observed band size: 63 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.