NARS Rabbit Polyclonal Antibody
cat.: HA500479
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 63 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human NARS aa 281-480 / 548. |
| Positive control: | HepG2 cell lysate, K562 cell lysate, 293 cell lysate, NIH/3T3 cell lysate, mouse liver tissue lysate, mouse brain tissue, mouse cerebellum tissue, rat cerebellum tissue. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB IHC-P |
1:1,000-1:5,000 1:600 |
| Uniprot #: | SwissProt: O43776 Human | Q8BP47 Mouse Entrez Gene: 291556 Rat |
| Alternative names: | 3010001M15Rik AA960128 AsnRS ASNS Asparagine tRNA ligase 1, cytoplasmic Asparagine tRNA ligase Asparagine--tRNA ligase Asparaginyl tRNA synthetase Asparaginyl-tRNA synthetase C78150 cytoplasmic EC 6.1.1.22 LRRGT00113 MGC116236 Nars NARS1 NRS SYNC_HUMAN |
Images
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Fig1:
Western blot analysis of NARS on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500479, 1/2,000) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: HepG2 cell lysate Lane 2: K562 cell lysate Lane 3: 293 cell lysate |
|
Fig2:
Western blot analysis of NARS on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500479, 1/1,000) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: NIH/3T3 cell lysate Lane 2: Mouse liver tissue lysate |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.