RNF111 Rabbit Polyclonal Antibody
cat.: HA500485
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 109 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human RNF111 aa 795-994 / 994. |
| Positive control: | K562 cell lysate, 293T cell lysate, Hela cell lysate, human placenta tissue, Hela. |
| Subcellular location: | Cytoplasm, Nucleus, PML body. |
| Recommended Dilutions:
WB IHC-P FC |
1:500-1:2,000 1:50-1:200 1:500-1:1,000 |
| Uniprot #: | SwissProt: Q6ZNA4 Human |
| Alternative names: | BCA2 Breast cancer associated gene 2 CL469780 E3 ubiquitin-protein ligase RNF115 Rabring 7 RING finger protein 115 RN115_HUMAN RNF115 Zinc finger protein 364 ZNF364 |
Images
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Fig1:
Western blot analysis of RNF111 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500485, 1/500) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: K562 cell lysate Lane 2: 293T cell lysate Lane 3: Hela cell lysate |
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Fig2: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-RNF111 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500485, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Flow cytometric analysis of Hela cells labeling RNF111. Cells were fixed and permeabilized. Then stained with the primary antibody (HA500485, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.