VGLUT1 Rabbit Polyclonal Antibody
cat.: HA500488
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 62 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human VGluT1 aa 1-63 / 560. |
| Positive control: | K562 cell lysates, mouse brain tissue lysate, rat brain tissue lysate, mouse cerebellum tissue lysate. |
| Subcellular location: | Synaptic vesicle membrane, synaptosome. |
| Recommended Dilutions:
WB |
1:500-1:2,000 |
| Uniprot #: | SwissProt: Q9P2U7 Human | Q3TXX4 Mouse | Q62634 Rat |
| Alternative names: | BNPI Brain specific Na (+) dependent inorganic phosphate cotransporter Brain specific Na dependent inorganic phosphate cotransporter Brain-specific Na(+)-dependent inorganic phosphate cotransporter Slc17a7 Solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 7 Solute carrier family 17 member 7 Vesicular glutamate transporter 1 VGLU1_HUMAN VGLUT 1 VGluT1 |
Images
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Fig1: Western blot analysis of VGluT1 on K562 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500488, 1/500) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of VGluT1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500488, 1/1,000) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Mouse brain tissue lysate Lane 2: Rat brain tissue lysate Lane 3: Mouse cerebellum tissue lysate |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.