WASP/Wiskott-Aldrich syndrome protein Rabbit Polyclonal Antibody
cat.: HA500490
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 53 kDa. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human WASP aa 1-200. |
| Positive control: | Rat bone marrow tissue lysates, Jurkat cell lysate, Daudi cell lysate, human spleen tissue, human tonsil tissue. |
| Subcellular location: | Cytoskeleton, Nucleus. |
| Recommended Dilutions:
WB IHC-P |
1:500-1:1,000 1:600 |
| Uniprot #: | SwissProt: P42768 Human Entrez Gene: 317371 Rat |
| Alternative names: | Eczema thrombocytopenia IMD2 SCNX THC THC1 Thrombocytopenia 1 (X linked) U42471 Was WASp WASP_HUMAN Wiskott Aldrich syndrome (eczema thrombocytopenia) Wiskott Aldrich syndrome Wiskott Aldrich syndrome protein Wiskott-Aldrich syndrome protein |
Images
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Fig1: Western blot analysis of WASP/Wiskott-Aldrich syndrome protein on rat bone marrow tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500490, 1/1,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of WASP/Wiskott-Aldrich syndrome protein on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500490, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Jurkat cell lysate Lane 2: Daudi cell lysate |
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Fig3: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-WASP/Wiskott-Aldrich syndrome protein antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500490, 1/600) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-WASP/Wiskott-Aldrich syndrome protein antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500490, 1/600) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.