WASP/Wiskott-Aldrich syndrome protein Rabbit Polyclonal Antibody
cat.: HA500490
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 53 kDa. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human WASP aa 1-200. |
| Positive control: | Rat bone marrow tissue lysates, Jurkat cell lysate, Daudi cell lysate, human spleen tissue, human tonsil tissue. |
| Subcellular location: | Cytoskeleton, Nucleus. |
| Recommended Dilutions:
WB IHC-P |
1:500-1:1,000 1:600 |
| Uniprot #: | SwissProt: P42768 Human Entrez Gene: 317371 Rat |
| Alternative names: | Eczema thrombocytopenia IMD2 SCNX THC THC1 Thrombocytopenia 1 (X linked) U42471 Was WASp WASP_HUMAN Wiskott Aldrich syndrome (eczema thrombocytopenia) Wiskott Aldrich syndrome Wiskott Aldrich syndrome protein Wiskott-Aldrich syndrome protein |
Images
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Fig1:
Western blot analysis of WASP/Wiskott-Aldrich syndrome protein on HeLa (Human cervical adenocarcinoma cell) lysates with Rabbit anti-WASP/Wiskott-Aldrich syndrome protein antibody (HA500490) at 1/2,000 dilution. Lysates/proteins at 15 µg/Lane. Exposure time: 59 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA500490, 1/2,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 52.9 kDa Observed band size: 60 kDa |
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Fig2: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-WASP/Wiskott-Aldrich syndrome protein antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500490, 1/600) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-WASP/Wiskott-Aldrich syndrome protein antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500490, 1/600) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.