Myogenin Rabbit Polyclonal Antibody
cat.: HA500492
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 25 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human Myogenin aa 1-200. |
| Positive control: | Human skeletal muscle tissue lysate, mouse smooth muscle tissue lysate, rat smooth muscle tissue lysate, rat skeletal muscle tissue lysate, rat kidney tissue. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB IHC-P |
1:1,000 1:600 |
| Uniprot #: | SwissProt: P15173 Human | P12979 Mouse | P20428 Rat |
| Alternative names: | bHLHc3 cb553 Class C basic helix-loop-helix protein 3 MYF 4 Myf-4 MYF4 MYOG MYOG_HUMAN Myogenic factor 4 Myogenic factor 4 myogenin Myogenin Myogenin myogenin factor 4 OTTHUMP00000039094 |
Images
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Fig1:
Western blot analysis of Myogenin on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500492, 1/1,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Human skeletal muscle tissue lysate Lane 2: Mouse smooth muscle tissue lysate Lane 3: Rat smooth muscle tissue lysate Lane 4: Rat skeletal muscle tissue lysate |
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Fig2: Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-Myogenin antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500492, 1/600) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.