CNG3 Rabbit Polyclonal Antibody
cat.: HA500493
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 79 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human CNG3 aa 503-694. |
| Positive control: | Rat brain tissue, CAL-62 cell lysates. |
| Subcellular location: | Membrane. |
| Recommended Dilutions:
WB IHC-P |
1:1,000 1:200 |
| Uniprot #: | SwissProt: Q16281 Human Entrez Gene: 85257 Rat |
| Alternative names: | ACHM2 CCNC1 CCNCa CCNCalpha CNCG3 CNG 3 CNG channel alpha 3 CNG channel alpha-3 CNG-3 CNG3 CNGA3 Cnga3 cyclic nucleotide gated channel alpha 3 CNGA3_HUMAN Cone photoreceptor cGMP gated channel Cone photoreceptor cGMP gated channel alpha subunit Cone photoreceptor cGMP gated channel subunit alpha Cone photoreceptor cGMP-gated channel subunit alpha Cyclic nucleotide gated cation channel alpha 3 Cyclic nucleotide gated channel alpha 3 Cyclic nucleotide gated channel olfactory 3 Cyclic nucleotide gated channel rod photoreceptor subunit alpha Cyclic nucleotide-gated cation channel alpha-3 Cyclic nucleotide-gated channel alpha-3 OTTHUMP00000161120 |
Images
|
Fig1: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-CNG3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500493, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig2:
Western blot analysis of CNG3 on CAL-62 cell lysate with Rabbit anti-CNG3 antibody (HA500493) at 1/1,000 dilution. Lysates/proteins at 10 µg/Lane. Exposure time: 60 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA500493, 1/1,000 in 5% NFDM/TBST, overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 79 kDa Observed band size: 79 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.