CNG3 Rabbit Polyclonal Antibody
cat.: HA500493
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 79 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human CNG3 aa 503-694. |
| Positive control: | CAL-62 cell lysates, rat brain tissue. |
| Subcellular location: | Membrane. |
| Recommended Dilutions:
WB IHC-P |
1:1,000 1:200 |
| Uniprot #: | SwissProt: Q16281 Human Entrez Gene: 85257 Rat |
| Alternative names: | ACHM2 CCNC1 CCNCa CCNCalpha CNCG3 CNG 3 CNG channel alpha 3 CNG channel alpha-3 CNG-3 CNG3 CNGA3 Cnga3 cyclic nucleotide gated channel alpha 3 CNGA3_HUMAN Cone photoreceptor cGMP gated channel Cone photoreceptor cGMP gated channel alpha subunit Cone photoreceptor cGMP gated channel subunit alpha Cone photoreceptor cGMP-gated channel subunit alpha Cyclic nucleotide gated cation channel alpha 3 Cyclic nucleotide gated channel alpha 3 Cyclic nucleotide gated channel olfactory 3 Cyclic nucleotide gated channel rod photoreceptor subunit alpha Cyclic nucleotide-gated cation channel alpha-3 Cyclic nucleotide-gated channel alpha-3 OTTHUMP00000161120 |
Images
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Fig1: Western blot analysis of CNG3 on CAL-62 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500493, 1/1,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. |
|
Fig2: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-CNG3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500493, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.