CYP1B1 Rabbit Polyclonal Antibody
cat.: HA500494
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 61 kDa. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human CYP1B1 aa 231-400. |
| Positive control: | A549 cell lysate, Hela cell lysate, rat skeletal muscle tissue. |
| Subcellular location: | Mitochondrion, Endoplasmic reticulum membrane, Microsome membrane. |
| Recommended Dilutions:
WB IHC-P |
1:500-1:1,000 1:50-1:200 |
| Uniprot #: | SwissProt: Q16678 Human | Q64678 Rat |
| Alternative names: | Aryl hydrocarbon hydroxylase CP1B CP1B1_HUMAN Cyp1b1 CYPIB1 Cytochrome P450 1B1 Cytochrome P450 family 1 subfamily B polypeptide 1 Cytochrome P450 subfamily I (dioxin inducible) polypeptide 1 (glaucoma 3 primary infantile) Flavoprotein linked monooxygenase GLC3A Microsomal monooxygenase P4501B1 Xenobiotic monooxygenase |
Images
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Fig1:
Western blot analysis of CYP1B1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500494, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: A549 cell lysate Lane 2: Hela cell lysate |
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Fig2: Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue using anti-CYP1B1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500494, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.