SOX17 Rabbit Polyclonal Antibody
cat.: HA500495
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse, Rat |
| Applications: | WB, IHC-P, IF-Tissue |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage buffer: | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 44 kDa. |
| Isotype: | IgG |
| Immunogen: | KLH conjugated synthetic peptide derived from Human SOX17 : 71-180/414 |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB IHC-P IF-Tissue |
1:500-1:2,000 1:100-1:500 1:100-1:500 |
| Uniprot #: | SwissProt: Q61473 Mouse Entrez Gene: 312936 Rat |
| Alternative names: | FLJ22252 SOX17 SOX17_HUMAN SRY (sex determining region Y) box 17 SRY box 17 SRY related HMG box transcription factor SOX17 Transcription factor SOX-17 Transcription factor SOX17 VUR3 |
Images
|
Fig1:
Sample: Lane 1: Mouse Heart tissue lysates Lane 2: Mouse Ovary tissue lysates Primary: Anti-SOX17 (HA500495) at 1/1,000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20,000 dilution Predicted band size: 44 kDa Observed band size: 48 kDa |
|
Fig2:
Tissue/cell: rat uterus tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-SOX17 Polyclonal Antibody, Unconjugated(HA500495) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.