TGF beta 1 Rabbit Polyclonal Antibody
cat.: HA500496
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 44 kDa.
Isotype: IgG
Immunogen: Recombinant protein within human TGF beta 1 aa 20-210.
Positive control: PC-3 cell lysate, A549 cell lysate, U87 cell lysate, Hela cell lysate, K562 cell lysate.
Subcellular location: Extracellular matrix, Secreted.
Recommended Dilutions:
  WB

1:500
Uniprot #: SwissProt: P01137 Human
Alternative names: Cartilage-inducing factor CED Differentiation inhibiting factor DPD1 LAP Latency-associated peptide Prepro transforming growth factor beta 1 TGF beta 1 TGF beta TGF beta 1 protein TGF-beta 1 protein TGF-beta-1 TGF-beta-5 TGF-beta1 TGFB Tgfb-1 tgfb1 TGFB1_HUMAN TGFbeta TGFbeta1 Transforming Growth Factor b1 Transforming Growth Factor beta 1 Transforming growth factor beta 1a transforming growth factor beta-1 transforming growth factor, beta 1 Transforming Growth Factor-ß1
Images
HA500496_1.jpg Fig1: Western blot analysis of TGF beta 1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500496, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: PC-3 cell lysate
Lane 2: A549 cell lysate
Lane 3: U87 cell lysate
Lane 4: Hela cell lysate
Lane 5: K562 cell lysate
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.