USP15 Rabbit Polyclonal Antibody
cat.: HA500497
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 112 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human USP15 aa 1-200. |
| Positive control: | Hela cell lysate, 293T cell lysate, NIH/3T3 cell lysate, human liver tissue, mouse liver tissue, rat liver tissue, K562. |
| Subcellular location: | Cytoplasm, Mitochondrion, Nucleus. |
| Recommended Dilutions:
WB IHC-P FC |
1:2,000 1:1,000 1:500-1:1,000 |
| Uniprot #: | SwissProt: Q9Y4E8 Human | Q8R5H1 Mouse | Q9R085 Rat |
| Alternative names: | Deubiquitinating enzyme 15 Deubiquitinating enzyme EC 3 1 2 15 KIAA0529 MGC131982 MGC149838 MGC74854 Ubiquitin Carboxy terminal Hydrolase 15 Ubiquitin carboxyl-terminal hydrolase 15 Ubiquitin specific peptidase 15 Ubiquitin specific processing protease 15 Ubiquitin Specific Protease 15 Ubiquitin thioesterase 15 Ubiquitin thiolesterase 15 Ubiquitin-specific-processing protease 15 UBP 15 UBP15 UBP15_HUMAN Unph 2 UNPH 4 Unph-2 Unph2 Unph4 USP 15 Usp15 |
Images
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Fig1:
Western blot analysis of USP15 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500497, 1/2,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Hela cell lysate Lane 2: 293T cell lysate Lane 3: NIH/3T3 cell lysate |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-USP15 antibody (HA500497) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500497) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-USP15 antibody (HA500497) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500497) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded rat liver tissue with Rabbit anti-USP15 antibody (HA500497) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500497) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig5:
Flow cytometric analysis of K562 cells labeling USP15. Cells were fixed and permeabilized. Then stained with the primary antibody (HA500497, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a Alexa Fluor® 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.