GSDMA Rabbit Polyclonal Antibody
cat.: HA500498
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Rat, Mouse |
| Applications: | WB |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 49 kDa. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human GSDMA aa 1-200. |
| Positive control: | COLO205 cell lysate,mouse skin tissue lysate, mouse stomach tissue lysate, rat skin tissue lysate. |
| Subcellular location: | Cytosol, perinuclear region; Cell membrane. |
| Recommended Dilutions:
WB |
1:5,000 |
| Uniprot #: | SwissProt: Q96QA5 Human Entrez Gene: 360619 Rat |
| Alternative names: | FKSG9 Gasdermin 1 Gasdermin A Gasdermin-1 Gasdermin-A GSDM GSDM1 GSDMA GSDMA_HUMAN |
Images
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Fig1:
Western blot analysis of GSDMA on different lysates with Rabbit anti-GSDMA antibody (HA500498) at 1/5,000 dilution. Lane 1: COLO205 cell lysate(15 µg/Lane) Lane 2: HeLa cell lysate(15 µg/Lane) Lane 3: mouse skin tissue lysate(30 µg/Lane) Lane 4: mouse stomach tissue lysate(30 µg/Lane) Lane 5: rat skin tissue lysate(30 µg/Lane) Exposure time: 12 seconds; ECL: K1801 Negative expression of GSDMA protein in HeLa cell is consistent with the predicted expression pattern. Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA500498, 1/5,000 in primary antibody dilution buffer 5% NFDM/TBST, overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 49 kDa Observed band size: 49 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.