Clathrin heavy chain Rabbit Polyclonal Antibody
cat.: HA500499
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 192 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human Clathrin heavy chain aa 1,401-1,550. |
| Positive control: | A431 cell lysate, mouse brain tissue lysate, human placenta tissue. |
| Subcellular location: | Spindle, Cytoplasmic vesicle membrane, Coated pit, Melanosome. |
| Recommended Dilutions:
WB IHC-P |
1:2,000 1:1,000 |
| Uniprot #: | SwissProt: Q00610 Human |
| Alternative names: | CLTC Clathrin heavy chain 1 Clathrin heavy chain on chromosome 17 CLH-17 CLH17 CLTCL2 KIAA0034 |
Images
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Fig1:
Western blot analysis of Clathrin heavy chain on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500499, 1/2,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: A431 cell lysate Lane 2: Mouse brain tissue lysate |
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Fig2: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-Clathrin heavy chain antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500499, 1/1,000) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.