DACT1 Rabbit Polyclonal Antibody
cat.: HA500500
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse |
| Applications: | WB, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 84 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human DACT1 aa 637-836. |
| Positive control: | Mouse testis tissue lysate, mouse liver tissue lysate, mouse cerebellum tissue. |
| Subcellular location: | Cytoplasm, nucleus, synapse. |
| Recommended Dilutions:
WB IHC-P |
1:500 1:600 |
| Uniprot #: | SwissProt: Q9NYF0 Human | Q8R4A3 Mouse |
| Alternative names: | Dact dact1 DACT1_HUMAN Dapper antagonist of beta catenin homolog 1 Dapper antagonist of catenin 1 Dapper Dapper homolog 1 DAPPER1 Dpr Dpr1 Frd Frodo hDPR1 Hepatocellular carcinoma novel gene 3 protein Heptacellular carcinoma novel gene 3 HNG3 MTNG3 Thyex3 |
Images
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Fig1:
Western blot analysis of DACT1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500500, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Mouse testis tissue lysate Lane 2: Mouse liver tissue lysate |
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Fig2: Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue using anti-DACT1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500500, 1/600) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.