DACT1 Rabbit Polyclonal Antibody
cat.: HA500500
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse |
| Applications: | WB, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 84 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human DACT1 aa 637-836. |
| Positive control: | Mouse liver tissue lysate, mouse testis tissue lysate, mouse brain tissue lysate, mouse cerebellum tissue. |
| Subcellular location: | Cytoplasm, nucleus, synapse. |
| Recommended Dilutions:
WB IHC-P |
1:5,000 1:600 |
| Uniprot #: | SwissProt: Q9NYF0 Human | Q8R4A3 Mouse |
| Alternative names: | Dact dact1 DACT1_HUMAN Dapper antagonist of beta catenin homolog 1 Dapper antagonist of catenin 1 Dapper Dapper homolog 1 DAPPER1 Dpr Dpr1 Frd Frodo hDPR1 Hepatocellular carcinoma novel gene 3 protein Heptacellular carcinoma novel gene 3 HNG3 MTNG3 Thyex3 |
Images
|
Fig1:
Western blot analysis of DACT1 on different lysates with Rabbit anti-DACT1 antibody (HA500500) at 1/5,000 dilution. Lane 1: Mouse liver tissue lysate Lane 2: Mouse testis tissue lysate Lane 3: Mouse brain tissue lysate Lysates/proteins at 15 µg/Lane. Exposure time: 25 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA500500, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 84 kDa Observed band size: 100 kDa |
|
Fig2: Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue using anti-DACT1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500500, 1/600) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.