GNB4 Rabbit Polyclonal Antibody
cat.: HA500501
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: 38 kDa
Isotype: IgG
Immunogen: Recombinant protein within human GNB4 aa 1-150.
Positive control: Human placenta tissue lysate, 293 cell lysate, A549 cell lysate, mouse brain lysates, rat brain lysates.
Subcellular location: Cytosol, extracellular exosome, lysosomal membrane, heterotrimeric G-protein complex, cytoplasm.
Recommended Dilutions:
  WB

1:1,000
Uniprot #: SwissProt: Q9HAV0 Human | P29387 Mouse | O35353 Rat
Alternative names: Guanine nucleotide-binding protein subunit beta-4 Transducin beta chain 4 GNB4
Images
HA500501_1.jpg Fig1: Western blot analysis of GNB4 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500501, 1/1,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Human placenta tissue lysate
Lane 2: 293 cell lysate
Lane 3: A549 cell lysate
HA500501_2.jpg Fig2: Western blot analysis of GNB4 on mouse brain lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500501, 1/1,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
HA500501_3.jpg Fig3: Western blot analysis of GNB4 on rat brain lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500501, 1/1,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.