P Glycoprotein Rabbit Polyclonal Antibody
cat.: HA500502
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 141 kDa. |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human P Glycoprotein aa 1,151-1,200. |
| Positive control: | Human brain tissue lysates, rat brain tissue, SH-SY5Y. |
| Subcellular location: | Cell membrane, Apical cell membrane. |
| Recommended Dilutions:
WB IHC-P FC |
1:5,000-1:15,000 1:1,000 1:50-1:200 |
| Uniprot #: | SwissProt: P08183 Human | P06795 Mouse | P43245 Rat |
| Alternative names: | ABC20 ABCB1 ATP binding cassette, sub family B (MDR/TAP), member 1 ATP-binding cassette sub-family B member 1 CD243 CLCS Colchicin sensitivity Doxorubicin resistance GP170 MDR1 MDR1_HUMAN Multidrug resistance 1 Multidrug resistance protein 1 P glycoprotein 1 P gp P-glycoprotein 1 PGY1 |
Images
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Fig1:
Western blot analysis of P Glycoprotein on human brain tissue lysate with Rabbit anti-P Glycoprotein antibody (HA500502) at 1/15,000 dilution. Lysates/proteins at 20 µg/Lane. Exposure time: 46 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA500502, 1/15,000 in 5% NFDM/TBST, 2 hours at room temperature Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 141 kDa Observed band size: 150 kDa |
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Fig2: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-P Glycoprotein antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500502, 1/1,000) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3: Flow cytometric analysis of P Glycoprotein was done on SH-SY5Y cells. The cells were fixed, permeabilized and stained with the primary antibody (HA500502, 1ug/ml) (red) compared with Rabbit IgG, monoclonal - Isotype Control ( green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.