SOX10 Rabbit Polyclonal Antibody
cat.: HA500503
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 50 kDa. |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human SOX10 aa 417-466. |
| Subcellular location: | Cytoplasm, Nucleus, Mitochondrion outer membrane. |
| Recommended Dilutions:
WB IHC-P |
1:2,000 1:600 |
| Uniprot #: | SwissProt: P56693 Human | Q04888 Mouse |
| Alternative names: | DOM DOM Dominant megacolon mouse human homolog of MGC15649 PCWH SOX 10 SOX10 SOX10_HUMAN SRY (sex determining region Y) box 10 SRY (sex determining region Y) box 10 SRY box 10 SRY box containing gene 10 SRY related HMG box gene 10 SRY related HMG box gene 10 Transcription factor SOX 10 Transcription factor SOX-10 WS2E WS4 WS4C |
Images
|
Fig1: Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue using anti-SOX10 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500503, 1/600) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig2:
Western blot analysis of SOX10 on different lysates with Rabbit anti-SOX10 antibody (HA500503) at 1/1,000 dilution. Lane 1: SK-MEL-28 (Human malignant melanoma cells) cell lysate Lane 2: B16-F1 (Mouse melanoma cells) cell lysate Lysates/proteins at 20 µg/Lane. Exposure time: 20 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA500503, 1/1,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 49.9 kDa Observed band size: 55 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.