HA500506

CNTFR Rabbit Polyclonal Antibody

cat.: HA500506

Product Type:	Rabbit polyclonal IgG, primary antibodies
Species reactivity:	Human, Mouse, Rat
Applications:	WB, IF-Cell, IHC-P, FC
Clonality:	Polyclonal

Form:	Liquid
Storage condition:	Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer:	1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration:	1ug/ul
Purification:	Immunogen affinity purified.
Molecular weight:	Predicted band size: 41 kDa.
Isotype:	IgG
Immunogen:	Synthetic peptide within human CNTFR aa 201-250.
Positive control:	Mouse cerebellum tissue lysate, rat brain tissue lysate, NIH/3T3 cell lysate, human fetal skeletal muscle tissue, mouse heart tissue, Hela, SH-SY5Y.
Subcellular location:	Cell membrane.
Recommended Dilutions: WB IF-Cell IHC-P FC	1:1,000 1:200 1:200 1:500-1:1,000
Uniprot #:	SwissProt: P26992 Human \| O88507 Mouse \| Q08406 Rat
Alternative names:	Ciliary neurotrophic factor receptor alpha Ciliary neurotrophic factor receptor Ciliary neurotrophic factor receptor subunit alpha CNTF Receptor alpha CNTF receptor subunit alpha CNTFR Alpha CNTFR CNTFR-alpha CNTFR_HUMAN

Images

	Fig1: Western blot analysis of CNTFR on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500506, 1/1,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Mouse cerebellum tissue lysate Lane 2: Rat brain tissue lysate Lane 3: NIH/3T3 cell lysate Predicted band size: 41 kDa Observed band size: 60 kDa (Glycosylation)
	Fig2: Immunohistochemical analysis of paraffin-embedded human fetal skeletal muscle tissue using anti-CNTFR antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA500506, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig3: Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-CNTFR antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA500506, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig4: ICC staining of CNTFR in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (HA500506, 1/200) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

Fig5: Flow cytometric analysis of SH-SY5Y cells labeling CNTFR.

Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA500506, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a Alexa Fluor® 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.