CRLF2 Rabbit Polyclonal Antibody
cat.: HA500507
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 42 kDa. |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human CRLF2 aa 301-350. |
| Positive control: | THP-1 cell lysate, HepG2 cell lysate, Jurkat cell lysate, 293 cell lysate, human endometrium tissue, A431, Hela. |
| Subcellular location: | Cell membrane; Secreted. |
| Recommended Dilutions:
WB IF-Cell IHC-P |
1:1,000 1:200 1:600 |
| Uniprot #: | SwissProt: Q9HC73 Human |
| Alternative names: | CRL 2 CRL2 CRLF 2 Crlf2 CRLF2_HUMAN CRLF2Y Cytokine receptor CRL2 precusor Cytokine receptor like 2 Cytokine receptor like factor 2 Cytokine receptor like factor 2 precursor Cytokine receptor-like 2 Cytokine receptor-like factor 2 IL XR IL-XR ILXR Thymic stromal derived lymphopoietin receptor Thymic stromal lymphopoietin protein receptor Thymic stromal lymphopoietin receptor TSLP receptor TSLPR |
Images
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Fig1:
Western blot analysis of CRLF2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500507, 1/1,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: THP-1 cell lysate Lane 2: HepG2 cell lysate Lane 3: Jurkat cell lysate Lane 4: 293 cell lysate Predicted band size: 42 kDa Observed band size: 48 kDa |
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Fig2: Immunohistochemical analysis of paraffin-embedded human endometrium tissue using anti-CRLF2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500507, 1/600) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3: ICC staining of CRLF2 in A431 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (HA500507, 1/200) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
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Fig4: ICC staining of CRLF2 in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (HA500507, 1/200) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.