Villin1 Rabbit Polyclonal Antibody
cat.: HA500509
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 93 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human Villin1 aa 778-827. |
| Positive control: | Human kidney tissue lysate, 293 cell lysate, mouse colon tissue lysate, rat kidney tissue lysate, rat colon tissue lysate, human kidney tissue, human small intestine tissue. |
| Subcellular location: | Cytoskeleton, lamellipodium, ruffle, microvillus, filopodium tip, filopodium. |
| Recommended Dilutions:
WB IHC-P |
1:1,000-1:2,000 1:600 |
| Uniprot #: | SwissProt: P09327 Human | Q62468 Mouse Entrez Gene: 316521 Rat |
| Alternative names: | D2S1471 OTTHUMP00000164145 VIL VIL1 VILI_HUMAN Villin 1 Villin-1 Villin1 |
Images
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Fig1:
Western blot analysis of Villin1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500509, 1/1,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Human kidney tissue lysate Lane 2: 293 cell lysate |
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Fig2:
Western blot analysis of Villin1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500509, 1/2,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Mouse colon tissue lysate Lane 2: Rat kidney tissue lysate Lane 2: Rat colon tissue lysate |
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Fig3: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Villin1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500509, 1/600) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4: Immunohistochemical analysis of paraffin-embedded human small intestine tissue using anti-Villin1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500509, 1/600) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.