CLIC5 Rabbit Polyclonal Antibody
cat.: HA500510
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 28 kDa. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human CLIC5 aa 41-240 / 251 (Q9NZA1-2). |
| Positive control: | Mouse small intestine tissue lysate, rat kidney tissue lysate, mouse small intestine tissue, mouse heart tissue. |
| Subcellular location: | Cytoplasm, cytoskeleton, apical cell membrane, cell cortex, membrane; centrosome, golgi apparatus. |
| Recommended Dilutions:
WB IHC-P |
1:5,000 1:200 |
| Uniprot #: | SwissProt: Q9NZA1 Human | Q8BXK9 Mouse | Q9EPT8 Rat |
| Alternative names: | Chloride intracellular channel 5 Chloride intracellular channel 5A Chloride intracellular channel protein 5 CLIC 5 CLIC 5B CLIC5 CLIC5_HUMAN CLIC5B dJ447E21.4 FLJ90663 MST 130 MST130 MSTP 130 MSTP130 |
Images
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Fig1:
Western blot analysis of CLIC5 on different lysates with Rabbit anti-CLIC5 antibody (HA500510) at 1/5,000 dilution. Lane 1: Mouse small intestine tissue lysate Lane 2: Rat kidney tissue lysate Lysates/proteins at 30 µg/Lane. Exposure time: 10 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA500510, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 28 kDa Observed band size: 28 kDa |
|
Fig2:
Application: Immunohistochemistry (IHC-P) Species: Mouse Tissue: Small intestine Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA500510, 1/200, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
|
Fig3:
Application: Immunohistochemistry (IHC-P) Species: Mouse Tissue: Heart Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA500510, 1/200, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.