Sigma1-receptor Rabbit Polyclonal Antibody
cat.: HA500511
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 25 kDa. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human Sigma1-receptor aa 74-223. |
| Positive control: | 293T cell lysate, HepG2 cell lysate, mouse liver tissue lysate, human liver tissue, human placenta tissue, mouse brain tissue. |
| Subcellular location: | Cell junction, Cell membrane, Cell projection, Cytoplasmic vesicle, Endoplasmic reticulum, Lipid droplet, Membrane, Nucleus, Postsynaptic cell membrane, Synapse. |
| Recommended Dilutions:
WB IHC-P |
1:500 1:600-1:1,000 |
| Uniprot #: | SwissProt: Q99720 Human | O55242 Mouse |
| Alternative names: | Aging associated gene 8 protein Aging-associated gene 8 protein AL024364 hSigmaR1 mSigmaR1 opioid receptor, sigma 1 opioid receptor, sigma 1 isoform 1 RP23 167I12.6 SGMR1_HUMAN SIG 1R SIG-1R Sigma 1-type opioid receptor Sigma non-opioid intracellular receptor 1 sigma1 receptor Sigma1-receptor Sigma1R Sigmar1 SR BP SR-BP SR31747 binding protein SR31747-binding protein SRBP |
Images
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Fig1:
Western blot analysis of Sigma1-receptor on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500511, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: 293T cell lysate Lane 2: HepG2 cell lysate Lane 3: Mouse liver tissue lysate |
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Fig2: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Sigma1-receptor antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500511, 1/600) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-Sigma1-receptor antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500511, 1/600) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Sigma1-receptor antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500511, 1/600) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.