Gbeta5 Rabbit Polyclonal Antibody
cat.: HA500513
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 44 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human Gbeta5 aa 1-200. |
| Positive control: | HepG2 cell lysate, mouse brain tissue lysate, rat brain tissue lysate, rat brain tissue, Hela. |
| Subcellular location: | Membrane. |
| Recommended Dilutions:
WB IHC-P FC |
1:1,000-1:5,000 1:50-1:200 1:100-1:500 |
| Uniprot #: | SwissProt: O14775 Human | P62881 Mouse | P62882 Rat |
| Alternative names: | FLJ37457 FLJ43714 G protein beta 5 subunit G protein beta subunit 5L GB 5 GB5 GBB5_HUMAN Gbeta5 GNB 5 GNB5 Guanine nucleotide binding protein (G protein) beta 5 Guanine nucleotide binding protein beta 5 Guanine nucleotide binding protein beta 5 subunit Guanine nucleotide binding protein beta subunit 5L Guanine nucleotide binding protein subunit beta 5 Guanine nucleotide-binding protein subunit beta-5 Transducin beta chain 5 |
Images
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Fig1:
Western blot analysis of Gbeta5 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500513, 1/1,000) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: HepG2 cell lysate Lane 2: Mouse brain tissue lysate Lane 3: Rat brain tissue lysate Predicted band size: 43 kDa Observed band size: 40 kDa |
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Fig2: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Gbeta5 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500513, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3: Flow cytometric analysis of Gbeta5 was done on Hela cells. The cells were stained with the primary antibody (HA500513, 1ug/ml) (red) compared with Rabbit IgG, monoclonal - Isotype Control (green). After incubation of the primary antibody at at +4℃ for 1 hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 min at +4℃ (dark incubation).Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.