Gbeta5 Rabbit Polyclonal Antibody
cat.: HA500513
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 44 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human Gbeta5 aa 1-200. |
| Positive control: | HepG2 cell lysate, mouse brain tissue lysate, rat brain tissue lysate, rat brain tissue, Hela. |
| Subcellular location: | Membrane. |
| Recommended Dilutions:
WB IHC-P |
1:1,000-1:5,000 1:50-1:200 |
| Uniprot #: | SwissProt: O14775 Human | P62881 Mouse | P62882 Rat |
| Alternative names: | FLJ37457 FLJ43714 G protein beta 5 subunit G protein beta subunit 5L GB 5 GB5 GBB5_HUMAN Gbeta5 GNB 5 GNB5 Guanine nucleotide binding protein (G protein) beta 5 Guanine nucleotide binding protein beta 5 Guanine nucleotide binding protein beta 5 subunit Guanine nucleotide binding protein beta subunit 5L Guanine nucleotide binding protein subunit beta 5 Guanine nucleotide-binding protein subunit beta-5 Transducin beta chain 5 |
Images
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Fig1: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Gbeta5 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500513, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig2:
Western blot analysis of Gbeta5 on different lysates with Rabbit anti-Gbeta5 antibody (HA500513) at 1/5,000 dilution. Lane 1: mouse brain tissue lysate Lane 2: rat brain tissue lysate Lysates/proteins at 20 µg/Lane. Exposure time: 25 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA500513, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 44 kDa Observed band size: 40 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.