CSNK2A2 Rabbit Polyclonal Antibody
cat.: HA500514
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 41 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human CSNK2A2 aa 151-350. |
| Positive control: | A549 cell lysate, HeLa cell lysate, 293T cell lysate, NIH/3T3 cell lysate, Mouse brain tissue lysate, Rat brain tissue lysate. |
| Subcellular location: | Cytosol, nucleoplasm, nucleus, plasma membrane, acrosomal vesicle, chromatin, protein kinase CK2 complex. |
| Recommended Dilutions:
WB |
1:1,000-1:2,000 |
| Uniprot #: | SwissProt: P19784 Human | O54833 Mouse Entrez Gene: 307641 Rat |
| Alternative names: | Casein kinase 2 alpha prime polypeptide Casein kinase II alpha' chain Casein kinase II subunit alpha' CK II alpha' CK II CK2A2 CKII CSK22_HUMAN CSNK2A1 CSNK2A2 FLJ43934 |
Images
|
Fig1:
Western blot analysis of CSNK2A2 on different lysates with Rabbit anti-CSNK2A2 antibody (HA500514) at 1/1,000 dilution. Lane 1: A549-WT cell lysate Lane 2: A549-KD CSNK2A2 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 41 kDa Observed band size: 35 kDa Exposure time: 23 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500514) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of CSNK2A2 on different lysates with Rabbit anti-CSNK2A2 antibody (HA500514) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: 293T cell lysate (20 µg/Lane) Lane 3: NIH/3T3 cell lysate (20 µg/Lane) Lane 4: Mouse brain tissue lysate (40 µg/Lane) Lane 5: Rat brain tissue lysate (40 µg/Lane) Predicted band size: 41 kDa Observed band size: 37 kDa Exposure time: 6 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500514) at 1/1,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.