MTCO1 Rabbit Polyclonal Antibody
cat.: HA500517
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2.1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 57 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human MTCO1 (1-11)ggggs (118-140)ggggs(213-227)( 434-446)ggggs(479-513). |
| Positive control: | Hela cell lysate, THP-1 cell lysate, SK-Br-3 cell lysate, MCF-7 cell lysate, RAW264.7 cell lysate, human tonsil tissue, human colon tissue, mouse heart tissue. |
| Subcellular location: | Mitochondrion inner membrane |
| Recommended Dilutions:
WB IHC-P |
1:1,000 1:2,000-1:5,000 |
| Uniprot #: | SwissProt: P00395 Human | P00397 Mouse |
| Alternative names: | COI COX I COX1 COX1_HUMAN COXI Cytochrome c oxidase polypeptide I Cytochrome c oxidase subunit 1 Cytochrome C Oxidase subunit I Mitochondrially encoded cytochrome c oxidase I MT CO1 MT-CO1 MTCO 1 MTCO1 |
Images
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Fig1:
Western blot analysis of MTCO1 on different lysates with Rabbit anti-MTCO1 antibody (HA500517) at 1/1,000 dilution. Lane 1: Hela cell lysate Lane 2: THP-1 cell lysate Lane 3: SK-Br-3 cell lysate Lane 4: MCF-7 cell lysate Lane 5: RAW264.7 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 57 kDa Observed band size: 35 kDa Exposure time: 1 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500517) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-MTCO1 antibody (HA500517) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500517) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-MTCO1 antibody (HA500517) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500517) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded mouse heart tissue with Rabbit anti-MTCO1 antibody (HA500517) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500517) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.