TATA binding protein TBP Rabbit Polyclonal Antibody
cat.: HA500518
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 2ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 38 kDa
Isotype: IgG
Immunogen: Recombinant protein within human TBP aa 140-339 / 339.
Positive control: Hela cell lysate, HCT116 cell lysate, 293T cell lysate, HepG2 cell lysate, MCF-7 cell lysate, Jurkat cell lysate, C2C12 cell lysate, NIH/3T3 cell lysate, RAW264.7 cell lysate, mouse testis tissue lysate, rat testis tissue lysate, human bladder carcinoma tissue, human testis tissue, human colon tissue, mouse stomach tissue.
Subcellular location: Nucleus.
Recommended Dilutions:
  WB
  IHC-P
1:1,000
1:2,000
Uniprot #: SwissProt: P20226 Human | P29037 Mouse
Entrez Gene: 117526 Rat
Alternative names: GTF2D GTF2D1 HDL4 MGC117320 MGC126054 MGC126055 SCA17 TATA binding factor TATA box factor TATA sequence binding protein TATA sequence-binding protein TATA-binding factor TATA-box binding protein N-terminal domain TATA-box factor TATA-box-binding protein TBP TBP_HUMAN TFIID Transcription initiation factor TFIID TBP subunit
Images
HA500518_1.jpg Fig1: Western blot analysis of TATA binding protein TBP on different lysates with Rabbit anti-TATA binding protein TBP antibody (HA500518) at 1/1,000 dilution.

Lane 1: Hela cell lysate (20 µg/Lane)
Lane 2: HCT116 cell lysate (20 µg/Lane)
Lane 3: 293T cell lysate (20 µg/Lane)
Lane 4: HepG2 cell lysate (20 µg/Lane)
Lane 5: MCF-7 cell lysate (20 µg/Lane)
Lane 6: Jurkat cell lysate (20 µg/Lane)
Lane 7: C2C12 cell lysate (20 µg/Lane)
Lane 8: NIH/3T3 cell lysate (20 µg/Lane)
Lane 9: RAW264.7 cell lysate (20 µg/Lane)
Lane 10: Mouse testis tissue lysate (40 µg/Lane)
Lane 11: Rat testis tissue lysate (40 µg/Lane)

Predicted band size: 38 kDa
Observed band size: 40/36 kDa

Exposure time: 3 minutes;

12% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500518) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
HA500518_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded human bladder carcinoma tissue with Rabbit anti-TATA binding protein TBP antibody (HA500518) at 1/2,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500518) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA500518_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-TATA binding protein TBP antibody (HA500518) at 1/2,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500518) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA500518_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-TATA binding protein TBP antibody (HA500518) at 1/2,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500518) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA500518_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded mouse stomach tissue with Rabbit anti-TATA binding protein TBP antibody (HA500518) at 1/2,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500518) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.