NOTCH3 Rabbit Polyclonal Antibody
cat.: HA500519
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 2ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 244 kDa
Isotype: IgG
Immunogen: Recombinant protein within human NOTCH3 aa 2,022-2,321 / 2,321.
Positive control: Hela cell lysate, MCF-7 cell lysate, PANC-1 cell lysate, HUVEC cell lysate, Hela.
Subcellular location: Cell membrane; Nucleus.
Recommended Dilutions:
  WB
  FC

1:1,000
1ug/mL
Uniprot #: SwissProt: Q9UM47 Human
Alternative names: CADASIL CASIL NOTC3_HUMAN Notch 3 Notch 3 intracellular domain Notch homolog 3 Notch3
Images
HA500519_1.jpg Fig1: Western blot analysis of NOTCH3 on different lysates with Rabbit anti-NOTCH3 antibody (HA500519) at 1/1,000 dilution.

Lane 1: Hela cell lysate
Lane 2: MCF-7 cell lysate
Lane 3: PANC-1 cell lysate
Lane 4: HUVEC cell lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 244 kDa
Observed band size: 90 kDa

Exposure time: 1 minute 30 seconds;

6% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500519) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
HA500519_2.jpg Fig2: Flow cytometric analysis of Hela cells labeling NOTCH3.

Cells were fixed and permeabilized. Then stained with the primary antibody (HA500519, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.