NOTCH3 Rabbit Polyclonal Antibody
cat.: HA500519
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 244 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human NOTCH3 aa 2,022-2,321 / 2,321. |
| Positive control: | Hela cell lysate, MCF-7 cell lysate, PANC-1 cell lysate, HUVEC cell lysate, Hela. |
| Subcellular location: | Cell membrane; Nucleus. |
| Recommended Dilutions:
WB FC |
1:1,000 1ug/mL |
| Uniprot #: | SwissProt: Q9UM47 Human |
| Alternative names: | CADASIL CASIL NOTC3_HUMAN Notch 3 Notch 3 intracellular domain Notch homolog 3 Notch3 |
Images
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Fig1:
Western blot analysis of NOTCH3 on different lysates with Rabbit anti-NOTCH3 antibody (HA500519) at 1/1,000 dilution. Lane 1: Hela cell lysate Lane 2: MCF-7 cell lysate Lane 3: PANC-1 cell lysate Lane 4: HUVEC cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 244 kDa Observed band size: 90 kDa Exposure time: 1 minute 30 seconds; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500519) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Flow cytometric analysis of Hela cells labeling NOTCH3. Cells were fixed and permeabilized. Then stained with the primary antibody (HA500519, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.