CCPG1 Rabbit Polyclonal Antibody
cat.: HA500520
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 87 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human CCPG1 aa 241-390 / 757. |
| Positive control: | HCT116 cell lysate, A549 cell lysate, Hela cell lysate, THP-1 cell lysate, SW480 cell lysate, HEK-293 cell lysate, NIH/3T3 cell lysate, C2C12 cell lysate, MEF cell lysate, SW480. |
| Subcellular location: | Cytoplasmic granule membrane. |
| Recommended Dilutions:
WB FC |
1:1,000 1ug/mL |
| Uniprot #: | SwissProt: Q9ULG6 Human | Q640L3 Mouse |
| Alternative names: | CCPG1 CCPG1_HUMAN Cell cycle progression 1 Cell cycle progression protein 1 Cell cycle progression restoration protein 8 CPR8 |
Images
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Fig1:
Western blot analysis of CCPG1 on different lysates with Rabbit anti-CCPG1 antibody (HA500520) at 1/1,000 dilution. Lane 1: HCT116 cell lysate Lane 2: A549 cell lysate Lane 3: Hela cell lysate Lane 4: THP-1 cell lysate Lane 5: SW480 cell lysate Lane 6: HEK-293 cell lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 87 kDa Observed band size: 105 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500520) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of CCPG1 on different lysates with Rabbit anti-CCPG1 antibody (HA500520) at 1/1,000 dilution. Lane 1: NIH/3T3 cell lysate Lane 2: C2C12 cell lysate Lane 3: MEF cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 87 kDa Observed band size: 105 kDa Exposure time: 6 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500520) at 1/1,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig3:
Flow cytometric analysis of SW480 cells labeling CCPG1. Cells were fixed and permeabilized. Then stained with the primary antibody (HA500520, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.