CCPG1 Rabbit Polyclonal Antibody
cat.: HA500520
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 2ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 87 kDa
Isotype: IgG
Immunogen: Recombinant protein within human CCPG1 aa 241-390 / 757.
Positive control: HCT116 cell lysate, A549 cell lysate, Hela cell lysate, THP-1 cell lysate, SW480 cell lysate, HEK-293 cell lysate, SW480.
Subcellular location: Cytoplasmic granule membrane.
Recommended Dilutions:
  WB
  FC

1:1,000
1ug/mL
Uniprot #: SwissProt: Q9ULG6 Human
Alternative names: CCPG1 CCPG1_HUMAN Cell cycle progression 1 Cell cycle progression protein 1 Cell cycle progression restoration protein 8 CPR8
Images
HA500520_1.jpg Fig1: Western blot analysis of CCPG1 on different lysates with Rabbit anti-CCPG1 antibody (HA500520) at 1/1,000 dilution.

Lane 1: HCT116 cell lysate
Lane 2: A549 cell lysate
Lane 3: Hela cell lysate
Lane 4: THP-1 cell lysate
Lane 5: SW480 cell lysate
Lane 6: HEK-293 cell lysate

Lysates/proteins at 30 µg/Lane.

Predicted band size: 87 kDa
Observed band size: 105 kDa

Exposure time: 2 minutes;

8% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500520) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
HA500520_2.jpg Fig2: Flow cytometric analysis of SW480 cells labeling CCPG1.

Cells were fixed and permeabilized. Then stained with the primary antibody (HA500520, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.