CCPG1 Rabbit Polyclonal Antibody
cat.: HA500520
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 87 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human CCPG1 aa 241-390 / 757. |
| Positive control: | HCT116 cell lysate, A549 cell lysate, Hela cell lysate, THP-1 cell lysate, SW480 cell lysate, HEK-293 cell lysate, NIH/3T3 cell lysate, C2C12 cell lysate, MEF cell lysate, SW480. |
| Subcellular location: | Cytoplasmic granule membrane. |
| Recommended Dilutions:
WB FC |
1:1,000 1ug/mL |
| Uniprot #: | SwissProt: Q9ULG6 Human | Q640L3 Mouse |
| Alternative names: | CCPG1 CCPG1_HUMAN Cell cycle progression 1 Cell cycle progression protein 1 Cell cycle progression restoration protein 8 CPR8 |
Images
|
Fig1:
Flow cytometric analysis of SW480 cells labeling CCPG1. Cells were fixed and permeabilized. Then stained with the primary antibody (HA500520, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
|
Fig2:
Western blot analysis of CCPG1 on different lysates with Rabbit anti-CCPG1 antibody (HA500520) at 1/1,000 dilution. Lane 1: A549(Human lung cancer cells) cell lysate Lane 2: HeLa(Human cervical cancer cells) cell lysate Lane 3: THP-1(Human acute monocytic leukemia cells) cell lysate Lane 4: SW480(Human colorectal adenocarcinoma cells) cell lysate Lane 5: HEK-293(Human embryonic kidney cell epithelioid cells) cell lysate Exposure time: 120 seconds; ECL: K1801 Negative expression of CCPG1 protein in mouse heart tissue is consistent with the predicted expression pattern. Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA500520, 1/1,000 in primary antibody dilution buffer , overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/300,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 87 kDa Observed band size: 105 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.